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Thermo Fisher sirna-notch1

The GLGZG suppressed the Notch signaling pathway in LPS-treated BV2 cells. In (A) by using real-time PCR the mRNA expressions of <t>Notch1,</t> NICD, RBPSUH, and HES1 were determined. In (B) the protein content of Notch1, NICD, RBPSUH, and HES1 in whole cells was also determined by Western blot assay. In (C) the protein levels of Notch1, NICD, and RBPSUH were ascertained by Western blot assay for cells transfected with siRNA-Notch1. Data are presented as means ± SD from three independent experiments done in triplicate. * P <0.05, ** P <0.01 vs. Control, # P <0.05, ## P <0.01 vs. LPS.

Sirna Notch1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirna-notch1 - by Bioz Stars, 2026-10

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1) Product Images from "Gualou Guizhi Granule inhibits microglia-mediated neuroinflammation to protect against neuronal apoptosis in vitro and in vivo"

Article Title: Gualou Guizhi Granule inhibits microglia-mediated neuroinflammation to protect against neuronal apoptosis in vitro and in vivo

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2024.1527986

The GLGZG suppressed the Notch signaling pathway in LPS-treated BV2 cells. In (A) by using real-time PCR the mRNA expressions of Notch1, NICD, RBPSUH, and HES1 were determined. In (B) the protein content of Notch1, NICD, RBPSUH, and HES1 in whole cells was also determined by Western blot assay. In (C) the protein levels of Notch1, NICD, and RBPSUH were ascertained by Western blot assay for cells transfected with siRNA-Notch1. Data are presented as means ± SD from three independent experiments done in triplicate. * P <0.05, ** P <0.01 vs. Control, # P <0.05, ## P <0.01 vs. LPS.

Figure Legend Snippet: The GLGZG suppressed the Notch signaling pathway in LPS-treated BV2 cells. In (A) by using real-time PCR the mRNA expressions of Notch1, NICD, RBPSUH, and HES1 were determined. In (B) the protein content of Notch1, NICD, RBPSUH, and HES1 in whole cells was also determined by Western blot assay. In (C) the protein levels of Notch1, NICD, and RBPSUH were ascertained by Western blot assay for cells transfected with siRNA-Notch1. Data are presented as means ± SD from three independent experiments done in triplicate. * P <0.05, ** P <0.01 vs. Control, # P <0.05, ## P <0.01 vs. LPS.

Techniques Used: Real-time Polymerase Chain Reaction, Western Blot, Transfection, Control

The influence exerted by the Notch-specific inhibitor and siRNA-Notch1 on GLGZG-inhibited microglial activation and pro-inflammatory factors production is shown. The BV2 cells were either pretreated with DAPT or transfected with siRNA-Notch1. Next, GLGZG was used to treat the cells, after which 24-hour LPS stimulation was given. In (A) are listed the IL-6 and TNF-α levels in culture medium measured by ELISA, upon DAPT-pretreated cells. In (B, C) are shown the protein content of the microglia activation of CD16 and CD32 upon DAPT-pretreated cells. In (D) the IL-6 and TNF-α levels in the culture medium were determined by ELISA upon the cells transfected with siRNA-Notch1. In (E, F) the protein content is revealed of the microglia activation of CD16 and CD32, upon cells transfected with siRNA-Notch1. Data are listed as means ± SD from three independent experiments performed in triplicate. ** P <0.01 vs. Control, # P <0.05, ## P <0.01 vs. LPS.

Figure Legend Snippet: The influence exerted by the Notch-specific inhibitor and siRNA-Notch1 on GLGZG-inhibited microglial activation and pro-inflammatory factors production is shown. The BV2 cells were either pretreated with DAPT or transfected with siRNA-Notch1. Next, GLGZG was used to treat the cells, after which 24-hour LPS stimulation was given. In (A) are listed the IL-6 and TNF-α levels in culture medium measured by ELISA, upon DAPT-pretreated cells. In (B, C) are shown the protein content of the microglia activation of CD16 and CD32 upon DAPT-pretreated cells. In (D) the IL-6 and TNF-α levels in the culture medium were determined by ELISA upon the cells transfected with siRNA-Notch1. In (E, F) the protein content is revealed of the microglia activation of CD16 and CD32, upon cells transfected with siRNA-Notch1. Data are listed as means ± SD from three independent experiments performed in triplicate. ** P <0.01 vs. Control, # P <0.05, ## P <0.01 vs. LPS.

Techniques Used: Activation Assay, Transfection, Enzyme-linked Immunosorbent Assay, Control

The effects of siRNA-Notch1 on GLGZG-inhibited HT22 cells with apoptosis stimulated by activated BV2 cells. In (A) is shown the cell viability of HT22, determined through CCK8 assay done for cells transfected with siRNA-Notch1. In (B) Western blot results for relative protein expression of Caspase-3 and Bax on cells transfected with siRNA-Notch1 are shown. Data are listed as means ± SD from three independent experiments conducted in triplicate. * P < 0.05, ** P < 0.01 vs Control, # P < 0.05, ## P < 0.01, vs LPS.

Figure Legend Snippet: The effects of siRNA-Notch1 on GLGZG-inhibited HT22 cells with apoptosis stimulated by activated BV2 cells. In (A) is shown the cell viability of HT22, determined through CCK8 assay done for cells transfected with siRNA-Notch1. In (B) Western blot results for relative protein expression of Caspase-3 and Bax on cells transfected with siRNA-Notch1 are shown. Data are listed as means ± SD from three independent experiments conducted in triplicate. * P < 0.05, ** P < 0.01 vs Control, # P < 0.05, ## P < 0.01, vs LPS.

Techniques Used: CCK-8 Assay, Transfection, Western Blot, Expressing, Control

Control:

Article Title: Notch1 is associated with the multidrug resistance of hypoxic osteosarcoma by regulating MRP1 gene expression.
Article Snippet: .. Notch1 siRNA (Invitrogen) or the scrambled control siRNA (Invitrogen) was transfected at the final concentration of 100 nM oligonucleotide using the Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s recommendations. ..

Article Title: Notch1 Signaling in FIZZ1 Induction of Myofibroblast Differentiation
Article Snippet: Human NIC and Jagged1 constructs pcDNA3.0-NIC and pcDNA3.1-Jagged1 expression plasmids were kind gifts from Dr. Lucio Miele (Cardinal Bernardin Cancer Center, Loyola University Medical Center, Maywood, IL) and Dr. Igor Prudovsky (Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, ME). .. Notch1 siRNA (5′-UGGCUUGCAGUAGCAAGGAAGCUAA-3′) and control siRNA (5′-UGGACGUGAUGGAACGAAGCUCUAA-3′) were self-designed and purchased from Invitrogen (Carlsbad, CA). .. Predesigned Jagged1 siRNA and its negative control siRNA were purchased from Ambion, Inc. (Austin, TX).

Article Title: Loss of Notch1 predisposes oro-esophageal epithelium to tumorigenesis.
Article Snippet: Notch signaling functions in diverse developmental and homeostatic processes, including stem cell self-renewal and cell fate determination.. Notch1-inactivating mutations are frequently detected in skin and oro-esophageal cancers, suggesting a role for Notch1 as a tumor suppressor.. Here, we clarify the contribution of Notch1 deficiency to oro-esophageal tumorigenesis using a physiological experimental model. Tongue and esophageal tumors induced in mice by 4-nitroquinoline-1-oxide (4-NQO) showed pathophysiological similarities to human tumors, including decreased Notch1 expression in the basal cells.

Transfection:

Article Title: Notch signaling modulates the activation of microglial cells.
Article Snippet: The oligonucleotides were purified by HPLC (Eurogentec, Belgium). .. Microglial cells were treated with the oligonucleotides at a final concentration of 5 lM during 48 h. Transfections with Notch1 siRNA (to a final concentration of 100 nM) were carried out in the six-well plates on primary microglia using Lipofectamine 2000 (Invitrogen) for 48 h. Notch1-specific siRNA duplex was 50- UUG AUG UCG AUC UCG CAG G and 50-CCU GCG AGA UCG ACA UCA A, and siRNA negative control duplex were ordered from Eurogentec. .. Total RNA was purified from cultured cells using the SV Total RNA Isolation System (Promega, CA).

Article Title: Defining super-enhancers by highly ranked histone H4 multi-acetylation levels identifies transcription factors associated with glioblastoma stem-like properties
Article Snippet: Colocalization was analyzed using NIS-Elements Ver4.30 (Nikon). .. For siRNA-mediated knockdown of gene expression, 0316-GSC, U87, and C13NJ cells were transfected with 25 nM siRNA targeting a gene of interest, negative control siRNA (Cat. No. AM4611, ThermoFisher Scientific), or NOTCH1-siRNA (Applied Biosystems) by using Lipofectamine RNAiMAX Transfection Reagent (Cat. No. 13778030, ThermoFisher Scientific). ..

Concentration Assay:

Negative Control:

Knockdown:

Gene Expression:

Image Search Results

(A-D) Box and scatter plots showing bulk RNA-sequencing data for key Notch pathway genes across a panel of human prostate cancer cell lines (LNCaP, LNCaP-abl, C4-2, C4-2B, VCaP, 22RV1, PC3, DU145, H660, LASCPC1, LNCaP_42D). (A-B) NOTCH1 and NOTCH2 Gene Expression (receptor). (C) HES1 Gene Expression (downstream effector). (D) DLL3 Gene Expression (ligand). (E) Western blot analysis confirming the protein expression of NOTCH2, DLL3, and HES1 in selected cell lines (LNCaP/AR, PC3, gTP53/RB1-R, and LASCPC1), corroborating the gene expression trends observed in the bulk RNA-seq data. Actin serves as a loading control.

Journal: bioRxiv

Article Title: Graded Notch Signaling Functions as a Rheostat of Lineage Plasticity and Therapy Resistance in Prostate Cancer

doi: 10.1101/2025.11.23.690056

Figure Lengend Snippet: (A-D) Box and scatter plots showing bulk RNA-sequencing data for key Notch pathway genes across a panel of human prostate cancer cell lines (LNCaP, LNCaP-abl, C4-2, C4-2B, VCaP, 22RV1, PC3, DU145, H660, LASCPC1, LNCaP_42D). (A-B) NOTCH1 and NOTCH2 Gene Expression (receptor). (C) HES1 Gene Expression (downstream effector). (D) DLL3 Gene Expression (ligand). (E) Western blot analysis confirming the protein expression of NOTCH2, DLL3, and HES1 in selected cell lines (LNCaP/AR, PC3, gTP53/RB1-R, and LASCPC1), corroborating the gene expression trends observed in the bulk RNA-seq data. Actin serves as a loading control.

Article Snippet: A pool of siRNAs targeting human NOTCH1, NOTCH2, HES1, and a non-targeting control pool were used (MedChemExpress; HY-RS09445, HY-RS09448, HY-RS06134).

Techniques: RNA Sequencing, Gene Expression, Western Blot, Expressing, Control

(A, B) Relative gene expression of Notch pathway components and lineage markers in enzalutamide-resistant cell lines, (A) gTP53/RB1-R and (B) PC3, after siRNA knockdown of specific genes ( NOTCH1, NOTCH2, HES1 ) compared to non-targeting control (NC). Significance is denoted as *p < 0.05, **p < 0.01, ***p < 0.001.

Journal: bioRxiv

Article Title: Graded Notch Signaling Functions as a Rheostat of Lineage Plasticity and Therapy Resistance in Prostate Cancer

doi: 10.1101/2025.11.23.690056

Figure Lengend Snippet: (A, B) Relative gene expression of Notch pathway components and lineage markers in enzalutamide-resistant cell lines, (A) gTP53/RB1-R and (B) PC3, after siRNA knockdown of specific genes ( NOTCH1, NOTCH2, HES1 ) compared to non-targeting control (NC). Significance is denoted as *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: A pool of siRNAs targeting human NOTCH1, NOTCH2, HES1, and a non-targeting control pool were used (MedChemExpress; HY-RS09445, HY-RS09448, HY-RS06134).

Techniques: Gene Expression, Knockdown, Control

Hla H35A activates Notch signaling via ADAM10 in Fl-BMDCs. a Representative western blot (left) and quantification (right) of N1ICD, N2ICD, Notch3, or Notch4 in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). b Quantification of Hey1 , Hes1 , or Hes5 mRNA expression by qPCR in Fl-BMDCs after 6 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). c Quantification of Adam10 , Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). d TPMs of the Notch1 , Notch2 , Notch3 , or Notch4 genes in unstimulated Fl-BMDCs from bulk RNA-seq ( n = 3 per group) . e Quantification of Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs under homeostatic conditions ( n = 3 per group). Each data point indicates a biological replicate in ( a ) and ( d ). Each data point indicates a biological replicate derived from the average of three technical replicates in ( b , c and e ). The data are presented as the means ± s.e.m.s. Statistical significance was tested via one-way ANOVA followed by Tukey’s multiple comparisons test in ( a – e )

Journal: Signal Transduction and Targeted Therapy

Article Title: Alpha hemolysin enhances the immune response by modulating dendritic cell differentiation via ADAM10-Notch signaling

doi: 10.1038/s41392-025-02432-3

Figure Lengend Snippet: Hla H35A activates Notch signaling via ADAM10 in Fl-BMDCs. a Representative western blot (left) and quantification (right) of N1ICD, N2ICD, Notch3, or Notch4 in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). b Quantification of Hey1 , Hes1 , or Hes5 mRNA expression by qPCR in Fl-BMDCs after 6 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). c Quantification of Adam10 , Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). d TPMs of the Notch1 , Notch2 , Notch3 , or Notch4 genes in unstimulated Fl-BMDCs from bulk RNA-seq ( n = 3 per group) . e Quantification of Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs under homeostatic conditions ( n = 3 per group). Each data point indicates a biological replicate in ( a ) and ( d ). Each data point indicates a biological replicate derived from the average of three technical replicates in ( b , c and e ). The data are presented as the means ± s.e.m.s. Statistical significance was tested via one-way ANOVA followed by Tukey’s multiple comparisons test in ( a – e )

Article Snippet: For siRNA-mediated knockdown of ADAM10, Notch1, or Notch2, mouse ADAM10 siRNA (siADAM10), mouse Notch1 siRNA (siNotch1), mouse Notch2 siRNA (siNotch2), and a nontargeting siRNA negative control (siNC) were synthesized by GENEWIZ (Suzhou, China).

Techniques: Western Blot, Expressing, RNA Sequencing, Derivative Assay

Fig. 2 siRNA-PTX-NPs suppressed NOTCH1 and SF3B1 at both the mRNA and protein levels. NOTCH1 and SF3B1 mRNA and protein expression levels were measured using qRT-PCR (a, b) and colorimetric analysis after treatment with various therapeutic agents (c-d). The data were normalized using β-actin mRNA expression levels as an internal control. The 2 –ΔΔCt technique was exploited to compute the relative mRNA expression level of the targeted genes. P-values < 0.05 (*), P-values < 0.01 (**), and P-values < 0.001 (***)

Journal: Cancer cell international

Article Title: Advancing the therapeutic effectiveness of paclitaxel in chronic lymphocytic leukemia through the simultaneous inhibition of NOTCH1 and SF3B1.

doi: 10.1186/s12935-025-03702-4

Figure Lengend Snippet: Fig. 2 siRNA-PTX-NPs suppressed NOTCH1 and SF3B1 at both the mRNA and protein levels. NOTCH1 and SF3B1 mRNA and protein expression levels were measured using qRT-PCR (a, b) and colorimetric analysis after treatment with various therapeutic agents (c-d). The data were normalized using β-actin mRNA expression levels as an internal control. The 2 –ΔΔCt technique was exploited to compute the relative mRNA expression level of the targeted genes. P-values < 0.05 (*), P-values < 0.01 (**), and P-values < 0.001 (***)

Article Snippet: Specific human siRNA against NOTCH1, SF3B1, and control siRNA were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Expressing, Quantitative RT-PCR, Control

Fig. 4 Silencing of SF3B1 and NOTCH1 significantly enhances the sensitivity of CLL cells to PTX-induced apoptosis. ELISA method was used to determine the cell death after treatment of CLL cells with different therapeutic agents (a). The qRT-PCR technique measured the mRNA expression levels of Bcl-2 and Bax after treatment of both PBMCs and BMMCs with different therapeutic agents (b, c). P-values < 0.05 (*), P-values < 0.01 (**), and P-values < 0.001 (***)

Journal: Cancer cell international

Article Title: Advancing the therapeutic effectiveness of paclitaxel in chronic lymphocytic leukemia through the simultaneous inhibition of NOTCH1 and SF3B1.

doi: 10.1186/s12935-025-03702-4

Figure Lengend Snippet: Fig. 4 Silencing of SF3B1 and NOTCH1 significantly enhances the sensitivity of CLL cells to PTX-induced apoptosis. ELISA method was used to determine the cell death after treatment of CLL cells with different therapeutic agents (a). The qRT-PCR technique measured the mRNA expression levels of Bcl-2 and Bax after treatment of both PBMCs and BMMCs with different therapeutic agents (b, c). P-values < 0.05 (*), P-values < 0.01 (**), and P-values < 0.001 (***)

Article Snippet: Specific human siRNA against NOTCH1, SF3B1, and control siRNA were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing

Fig. 6 The competing endogenous RNA (ceRNA) network that modulates NOTCH1 and SF3B1 genes. The ceRNA network, including NOTCH1 and SF3B1, 9 miRNAs targeting both genes and 46 lncRNAs sponging selected miRNAs were constructed (a). The enrichment analysis underscored the pivotal path ways where the ceRNA network, associated with NOTCH1 and SF3B1, exerts its influence in the context of chronic lymphocytic leukemia (b)

Journal: Cancer cell international

Article Title: Advancing the therapeutic effectiveness of paclitaxel in chronic lymphocytic leukemia through the simultaneous inhibition of NOTCH1 and SF3B1.

doi: 10.1186/s12935-025-03702-4

Figure Lengend Snippet: Fig. 6 The competing endogenous RNA (ceRNA) network that modulates NOTCH1 and SF3B1 genes. The ceRNA network, including NOTCH1 and SF3B1, 9 miRNAs targeting both genes and 46 lncRNAs sponging selected miRNAs were constructed (a). The enrichment analysis underscored the pivotal path ways where the ceRNA network, associated with NOTCH1 and SF3B1, exerts its influence in the context of chronic lymphocytic leukemia (b)

Article Snippet: Specific human siRNA against NOTCH1, SF3B1, and control siRNA were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Construct

FIGURE 2 | Knockdown of Notch1 reduced TMZ resistance in GBM cells. (A) CCK8 analysis of TMZ treatment (0–2000 μM) up to 72 h in U87-R cells, (B) CCK8 analysis of TMZ treatment (0–2000 μM) up to 72 h in U251-R cells, (C) CCK-8 analysis in U87 and U87-R cells, (D) CCK-8 analysis in U87 and U87-R cells, (E) U87-R cells treated with 600 μM TMZ and siNotch1 for 48 h, (F) U251-R cells treated with 600 μM TMZ and siNotch1 for 48 h. Images in E, F were viewed with a 20× objective. *p < 0.05, **p < 0.001, and ***p < 0.0001 compared with control cells.

Journal: Journal of cellular and molecular medicine

Article Title: Notch1 and Major Vault Proteins Modulate Temozolomide Resistance in Glioblastoma.

doi: 10.1111/jcmm.70474

Figure Lengend Snippet: FIGURE 2 | Knockdown of Notch1 reduced TMZ resistance in GBM cells. (A) CCK8 analysis of TMZ treatment (0–2000 μM) up to 72 h in U87-R cells, (B) CCK8 analysis of TMZ treatment (0–2000 μM) up to 72 h in U251-R cells, (C) CCK-8 analysis in U87 and U87-R cells, (D) CCK-8 analysis in U87 and U87-R cells, (E) U87-R cells treated with 600 μM TMZ and siNotch1 for 48 h, (F) U251-R cells treated with 600 μM TMZ and siNotch1 for 48 h. Images in E, F were viewed with a 20× objective. *p < 0.05, **p < 0.001, and ***p < 0.0001 compared with control cells.

Article Snippet: We used siRNA molecules, specifically designed to target Notch1, with the sequence for siNotch1 (Santa Cruz Biotechnology) (sense 5′- UGU CAU CCA UCA GAA CUG GGG3′, and antisense 5′- CCU UGU GAC UUU UCG UAA UUA- 3′).

Techniques: Knockdown, CCK-8 Assay, Control

FIGURE 3 | Knockdown of Notch1 reduced the multiple resistance proteins levels in GBM cells. (A) ELISA analysis of Notch1 levels in TMZ- resistant and non-resistant GBM cells, (B) ELISA analysis of MGMT levels in TMZ-resistant and non-resistant GBM cells, (C) ELISA analysis of MVP levels in TMZ-resistant and non-resistant GBM cells, (D) ELISA analysis of ABCG2 levels in TMZ-resistant and non-resistant GBM cells.

Journal: Journal of cellular and molecular medicine

Article Title: Notch1 and Major Vault Proteins Modulate Temozolomide Resistance in Glioblastoma.

doi: 10.1111/jcmm.70474

Figure Lengend Snippet: FIGURE 3 | Knockdown of Notch1 reduced the multiple resistance proteins levels in GBM cells. (A) ELISA analysis of Notch1 levels in TMZ- resistant and non-resistant GBM cells, (B) ELISA analysis of MGMT levels in TMZ-resistant and non-resistant GBM cells, (C) ELISA analysis of MVP levels in TMZ-resistant and non-resistant GBM cells, (D) ELISA analysis of ABCG2 levels in TMZ-resistant and non-resistant GBM cells.

Techniques: Knockdown, Enzyme-linked Immunosorbent Assay

FIGURE 4 | RT-PCR and Western blot analyzes of multiple resistance proteins in Notch1 knockdown GBM cells. (A) RT-PCR and Western blot analysis of Notch1, MVP, ABCG2 and MGMT levels in U87 and U87-R cells, (B) RT-PCR and Western blot analysis of Notch1, MVP, ABCG2, and MGMT levels in U251 and U251-R cells. aaap < 0.0001 compared with TMZ-non-resistant GBM cells, bbp < 0.001 compared with TMZ-resistant GBM cells, and bbbp < 0.0001 compared with TMZ-resistant GBM cells.

Journal: Journal of cellular and molecular medicine

Article Title: Notch1 and Major Vault Proteins Modulate Temozolomide Resistance in Glioblastoma.

doi: 10.1111/jcmm.70474

Figure Lengend Snippet: FIGURE 4 | RT-PCR and Western blot analyzes of multiple resistance proteins in Notch1 knockdown GBM cells. (A) RT-PCR and Western blot analysis of Notch1, MVP, ABCG2 and MGMT levels in U87 and U87-R cells, (B) RT-PCR and Western blot analysis of Notch1, MVP, ABCG2, and MGMT levels in U251 and U251-R cells. aaap < 0.0001 compared with TMZ-non-resistant GBM cells, bbp < 0.001 compared with TMZ-resistant GBM cells, and bbbp < 0.0001 compared with TMZ-resistant GBM cells.

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Knockdown

FIGURE 5 | EMT induced in TMZ-resistant cells was reversed by Notch1 downregulation. (A) ELISA analysis of vimentin levels in TMZ-resistant and non-resistant GBM cells, (B) ELISA analysis of N-cadherin levels in TMZ-resistant and non-resistant GBM cells, (C) Western blot analysis of vimentin and N-cadherin protein levels in TMZ-resistant and non-resistant GBM cell, (D) ELISA analysis of vimentin levels in Notch1 knockdown U87-R and U251-R cells, (E) ELISA analysis of N-cadherin levels in Notch1 knockdown U87-R and U251-R cells, (F) Western blot analysis of vimen- tin and N-cadherin protein levels in Notch1 knockdown U87-R and U251-R cells. **p < 0.001, and ***p < 0.0001 compared with control cells.

Journal: Journal of cellular and molecular medicine

Article Title: Notch1 and Major Vault Proteins Modulate Temozolomide Resistance in Glioblastoma.

doi: 10.1111/jcmm.70474

Figure Lengend Snippet: FIGURE 5 | EMT induced in TMZ-resistant cells was reversed by Notch1 downregulation. (A) ELISA analysis of vimentin levels in TMZ-resistant and non-resistant GBM cells, (B) ELISA analysis of N-cadherin levels in TMZ-resistant and non-resistant GBM cells, (C) Western blot analysis of vimentin and N-cadherin protein levels in TMZ-resistant and non-resistant GBM cell, (D) ELISA analysis of vimentin levels in Notch1 knockdown U87-R and U251-R cells, (E) ELISA analysis of N-cadherin levels in Notch1 knockdown U87-R and U251-R cells, (F) Western blot analysis of vimen- tin and N-cadherin protein levels in Notch1 knockdown U87-R and U251-R cells. **p < 0.001, and ***p < 0.0001 compared with control cells.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Knockdown, Control

Journal: Frontiers in Immunology

Article Title: Gualou Guizhi Granule inhibits microglia-mediated neuroinflammation to protect against neuronal apoptosis in vitro and in vivo

doi: 10.3389/fimmu.2024.1527986

Figure Lengend Snippet: The GLGZG suppressed the Notch signaling pathway in LPS-treated BV2 cells. In (A) by using real-time PCR the mRNA expressions of Notch1, NICD, RBPSUH, and HES1 were determined. In (B) the protein content of Notch1, NICD, RBPSUH, and HES1 in whole cells was also determined by Western blot assay. In (C) the protein levels of Notch1, NICD, and RBPSUH were ascertained by Western blot assay for cells transfected with siRNA-Notch1. Data are presented as means ± SD from three independent experiments done in triplicate. * P <0.05, ** P <0.01 vs. Control, # P <0.05, ## P <0.01 vs. LPS.

Article Snippet: They were then transfected with siRNA-Notch1 utilizing LipofectamineTM 2000 (Invitrogen, Carlsbad, CA, USA) and incubated for 6 more hours.

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Transfection, Control

Journal: Frontiers in Immunology

Article Title: Gualou Guizhi Granule inhibits microglia-mediated neuroinflammation to protect against neuronal apoptosis in vitro and in vivo

doi: 10.3389/fimmu.2024.1527986

Figure Lengend Snippet: The influence exerted by the Notch-specific inhibitor and siRNA-Notch1 on GLGZG-inhibited microglial activation and pro-inflammatory factors production is shown. The BV2 cells were either pretreated with DAPT or transfected with siRNA-Notch1. Next, GLGZG was used to treat the cells, after which 24-hour LPS stimulation was given. In (A) are listed the IL-6 and TNF-α levels in culture medium measured by ELISA, upon DAPT-pretreated cells. In (B, C) are shown the protein content of the microglia activation of CD16 and CD32 upon DAPT-pretreated cells. In (D) the IL-6 and TNF-α levels in the culture medium were determined by ELISA upon the cells transfected with siRNA-Notch1. In (E, F) the protein content is revealed of the microglia activation of CD16 and CD32, upon cells transfected with siRNA-Notch1. Data are listed as means ± SD from three independent experiments performed in triplicate. ** P <0.01 vs. Control, # P <0.05, ## P <0.01 vs. LPS.

Article Snippet: They were then transfected with siRNA-Notch1 utilizing LipofectamineTM 2000 (Invitrogen, Carlsbad, CA, USA) and incubated for 6 more hours.

Techniques: Activation Assay, Transfection, Enzyme-linked Immunosorbent Assay, Control

Journal: Frontiers in Immunology

Article Title: Gualou Guizhi Granule inhibits microglia-mediated neuroinflammation to protect against neuronal apoptosis in vitro and in vivo

doi: 10.3389/fimmu.2024.1527986

Figure Lengend Snippet: The effects of siRNA-Notch1 on GLGZG-inhibited HT22 cells with apoptosis stimulated by activated BV2 cells. In (A) is shown the cell viability of HT22, determined through CCK8 assay done for cells transfected with siRNA-Notch1. In (B) Western blot results for relative protein expression of Caspase-3 and Bax on cells transfected with siRNA-Notch1 are shown. Data are listed as means ± SD from three independent experiments conducted in triplicate. * P < 0.05, ** P < 0.01 vs Control, # P < 0.05, ## P < 0.01, vs LPS.

Article Snippet: They were then transfected with siRNA-Notch1 utilizing LipofectamineTM 2000 (Invitrogen, Carlsbad, CA, USA) and incubated for 6 more hours.

Techniques: CCK-8 Assay, Transfection, Western Blot, Expressing, Control

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